Journal: Nucleic Acids Research

Article Title: Selective single cell isolation for genomics using microraft arrays

doi: 10.1093/nar/gkw700

Figure Lengend Snippet: Comparing RNA-seq from cells isolated with microrafts and the Fluidigm C1. ( A ) Fraction of reads aligning to the human genome. ( B ) Gene body coverage plots measuring 3′ bias in read coverage. ( C ) Distribution of read coverage by genomic region. ( D ) Number of genes detected. ( E ) Read coverage for empty samples. ( F ) Heatmap of Pearson correlations among C1 and microraft cells. ( G ) Heatmap of chromosome condensation gene set, which shows strong upregulation in raft cells. ( H ) Heatmap of cell junction gene set, which shows slight upregulation in C1 cells.

Article Snippet: Libraries were created using the C1 recommended Nextera XT kit with the C1 modifications for low concentration reactions.

Techniques: RNA Sequencing, Isolation

Journal: PLoS ONE

Article Title: Integrative Omics Analysis of Rheumatoid Arthritis Identifies Non-Obvious Therapeutic Targets

doi: 10.1371/journal.pone.0124254

Figure Lengend Snippet: ( A ) Western blots showing RAC1 and Rac1-GTP levels in RA FLS following PDGF stimulation with ELMO1 and control siRNA. RAC1 activation was determined as described in Material and Methods. Cells were assayed from 0 to 15 min after they were stimulated with PDGF. ( B ) Quantification of the RAC1 assay. Mean and SEM was calculated from 3 experiments. The ratio of Rac1 and Rac1-GTP in RA FLS following PDGF stimulation with ELMO1 and control siRNA is shown and demonstrates decreased RAC1 activation when FLS are ELMO1 -deficient.

Article Snippet: Activated RAC1 was detected were using RAC1 assay reagent (GST-PAK1-PBD on glutathione-Sepharose Beads) according to the manufacturer’s instructions (Cytoskeleton, Denver CO).

Techniques: Western Blot, Control, Activation Assay